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JoVE Journal
Developmental Biology

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将DNA微注射到种胚胎的眼蕾中,以及GFP表达光学轴状轴向轴向的成像,活的Xenopus大白鼠
 
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将DNA微注射到种胚胎的眼蕾中,以及GFP表达光学轴状轴向轴向的成像,活的Xenopus大白鼠

Article DOI: 10.3791/60123-v 06:32 min September 4th, 2019
September 4th, 2019

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Summary

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Click here for the English version.

该协议旨在演示如何将DNA/DOTAP混合物微注射到一天长的Xenopus laevis胚胎的眼蕾中,以及如何在中脑中图像和重建单个绿色荧光蛋白(GFP),表达光学轴向轴向。完好无损,活着的Xenopus小动物。

Tags

发育生物学, 第 151 期,异形杆菌 , 唇裂, 视轴轴, GFP, 成像, 显微注射, 眼蕾
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