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Journal / Developmental Biology / Mikroinjektion von DNA in Augenknospen in Xenopus …
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JoVE Journal Developmental Biology
Mikroinjektion von DNA in Augenknospen in Xenopus laevis Embryonen und Bildgebung von GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
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Mikroinjektion von DNA in Augenknospen in Xenopus laevis Embryonen und Bildgebung von GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Article DOI: 10.3791/60123-v • 06:32 min • September 4th, 2019
September 4th, 2019 •
1, 1, 1
1College of Osteopathic Medicine, Touro University California

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Dieses Protokoll soll zeigen, wie man ein DNA/DOTAP-Gemisch in Augenknospen von eintägigen Xenopus laevis Embryonen mikroinjiziert und wie man einzelne grüne fluoreszierende Proteine (GFP) abbilden und rekonstruieren kann, die optische axonale Lauben in tektalen Midhirnen von intakte, lebende Xenopus Kaulquappen.

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Entwicklungsbiologie Ausgabe 151 Xenopus laevis Lipofection optische axonale Lauben GFP Bildgebung Mikroinjektion Augenknospen
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