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Differentiering af mus bryst epitel HC11 og EpH4 Celler
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Differentiering af mus bryst epitel HC11 og EpH4 Celler

Article DOI: 10.3791/60147-v • 09:32 min • February 27th, 2020
February 27th, 2020 •
*1,2, *1, *1,3, 1,4, 1, 1, 2, 1
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, 2Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
* These authors contributed equally

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Vi beskriver teknikker til differentiering induktion af to bryst epitellinjer, HC11 og EpH4. Mens begge kræver føtal kalv serum, insulin, og prolaktin til at producere mælkeproteiner, EpH4 celler kan fuldt ud differentiere sig i mammsfærer i tre-dimensionel kultur. Disse komplementære modeller er nyttige til undersøgelser af signaltransduktion af differentiering og neoplasi.

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Kræftforskning celledifferentiering brystepitelceller prolaktin EHS-matrix
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