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Kıvılcım Susturma: CRISPR/Cas9 Zayıf Elektrikli Balıklarda Genom Düzenleme

Article DOI: 10.3791/60253-v • 08:00 min • October 27th, 2019
October 27th, 2019 •
1, 2, 2, 3, 1
1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University

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Burada CRISPR/Cas9 genom nakavt elektrikli balık üretimi ve arkası için bir protokol sunulmuştur. Ayrıntılı olarak özetlenen bir gymnotiform ve mormyrid hem de gerekli moleküler biyoloji, Üreme ve hayvancılık gereksinimleri ve cas9 indel F0 larvaları üretmek için enjeksiyon teknikleri vardır.

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Biyoloji Sayı 152 CRISPR/Cas9 elektrikli balık tek hücreli mikroenjeksiyon scn4aa,elektrik organı deşarjı genom modifikasyonu mormyrid gymnotiform
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