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Chapters
- 00:04Introduction
- 00:58Murine Flexor Digitorum Brevis Muscle Dissection
- 02:07Muscle Digestion, Trituration, and Gravity Sedimentation
- 02:59Fiber Embedding
- 03:44Optics‐Based Contractile Measurements
- 06:20Results: Embedded Muscle Contraction Assessment
- 06:59Conclusion
Skeletal muscle function can be assessed by quantifying the contractility of isolated muscle fibers, traditionally using laborious, low-throughput approaches. Here, we describe an optics-based, high-throughput method to quantify the contractility of hydrogel-embedded muscle fibers. This approach has applications for drug screening and therapeutic development.
