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Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
DOI:

10:23 min

December 14, 2019

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Chapters

  • 00:00Title
  • 00:53Live Cells Co-Culture Model of 4T1 Mouse Mammary Carcinomas Cells and Raw 264.7 Mouse Macrophages
  • 06:52Time-lapse Assessment of M1 Macrophages Phagocyted 4T1 Cells
  • 08:18Results
  • 09:49Conclusion

Summary

Automatic Translation

Macrophage phagocytic activity against cancer cells, specifically 4T1 mouse mammary carcinoma cells, was imaged in this study. The live cell coculture model was established and observed using a combination of fluorescent and differential interference contrast microscopy. This assessment was imaged using imaging software to develop multipoint time-lapse video.

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