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JoVE Journal Cancer Research
Time-lapse 2D-beeldvorming van fagocytische activiteit in M1 macrophage-4T1 muis borstkanker cellen in co-culturen
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Time-lapse 2D-beeldvorming van fagocytische activiteit in M1 macrophage-4T1 muis borstkanker cellen in co-culturen

Article DOI: 10.3791/60281-v • 10:23 min • December 14th, 2019
December 14th, 2019 •
1, 2, 1, 1, 3, 4, 1,2
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 2Institute of Tropical Forestry and Forestry Products (INTROP), Universiti Putra Malaysia, 3Department of Biomedical Science, Faculty of Medicine, University of Malaya, 4Agro-Biotechnology Institute (ABI), National Institute of Biotechnology Malaysia (NIBM)

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Macrofaag fagocytische activiteit tegen kankercellen, in het bijzonder 4T1 muis borstklier carcinoom cellen, werd afgebeeld in deze studie. Het cocultuurmodel van de levende cellen werd vastgesteld en waargenomen met behulp van een combinatie van fluorescerende en differentiële interferentie contrast microscopie. Deze beoordeling is gemaakt met behulp van imaging software om multipoint time-lapse-video te ontwikkelen.

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Kankeronderzoek afgifte 154 immunologie immuno-oncologie Kankerbiologie time-lapse microscopie Live-celbeeldvorming fagocytose macrofagen tumor borstkanker coculturen tumor micro
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