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Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins
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Neuroscience
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JoVE Journal Neuroscience
Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins

Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins

DOI:
10.3791/60760-v

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07:04 min

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February 07, 2020

•

,
1Kresge Hearing Research Institute, Department of Otolaryngology – Head and Neck Surgery,University of Michigan

Chapters

  • 00:04Introduction
  • 00:51Preparation for Surgery
  • 01:44Surgery
  • 04:47In Vitro Recording
  • 05:27Results: ChrimsonR is an Effective Red-shifted Opsin for Long-range CRACM Experiments in the Auditory Brainstem
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

Channelrhodopsin-assisted circuit mapping (CRACM) is a precision technique for functional mapping of long-range neuronal projections between anatomically and/or genetically identified groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem connections, including the use of a red-shifted opsin, ChrimsonR.

Tags

ChannelrhodopsinCircuit MappingInferior ColliculusBlue-shiftedRed-shiftedNeural ProjectionsAuditory Brain StemCRACMStereotaxicCraniotomyNano-injectorMicromanipulatorAnesthesiaTemperature ControlAnalgesicSurgery

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