Other Publications (1)
Articles by David Jelinek in JoVE
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue David Jelinek1,2, Aimee Flores1,3, Melanie Uebelhoer1, Vincent Pasque2, Kathrin Plath2,3, M. Luisa Iruela-Arispe1,3, Heather R. Christofk2,3, William E. Lowry1,3, Hilary A. Coller1,2,3 1Department of Molecular, Cell and Developmental Biology, UCLA, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, UCLA We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.
Other articles by David Jelinek on PubMed
Lactate Dehydrogenase Activity Drives Hair Follicle Stem Cell Activation Nature Cell Biology. Sep, 2017 | Pubmed ID: 28812580 Although normally dormant, hair follicle stem cells (HFSCs) quickly become activated to divide during a new hair cycle. The quiescence of HFSCs is known to be regulated by a number of intrinsic and extrinsic mechanisms. Here we provide several lines of evidence to demonstrate that HFSCs utilize glycolytic metabolism and produce significantly more lactate than other cells in the epidermis. Furthermore, lactate generation appears to be critical for the activation of HFSCs as deletion of lactate dehydrogenase (Ldha) prevented their activation. Conversely, genetically promoting lactate production in HFSCs through mitochondrial pyruvate carrier 1 (Mpc1) deletion accelerated their activation and the hair cycle. Finally, we identify small molecules that increase lactate production by stimulating Myc levels or inhibiting Mpc1 carrier activity and can topically induce the hair cycle. These data suggest that HFSCs maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli.