Journal
/
Biochemistry
/
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

DOI:
10.3791/57760-v

•

06:18 min

•

June 21, 2018

•

, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Chapters

  • 00:04Title
  • 00:31Generate Slides of Frozen Mouse Skin
  • 01:53Slides and Staining Solution Preparation
  • 02:54Staining and Imaging
  • 04:08Results: Representative Results of Lactate Dehydrogenase Staining
  • 05:43Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.

Tags

Keywords: Lactate DehydrogenaseEnzyme ActivityCryomoldCryosectioningHistochemical StainingNADPMSNitrotetrazolium Blue ChlorideLactateEnzyme Localization

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57760?article_type=v

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
check_url/57760?article_type=v

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
check_url/57760?article_type=v

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
check_url/57760?article_type=v

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
check_url/57760?article_type=v

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
check_url/57760?article_type=v

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
check_url/57760?article_type=v

Quantification of Hypopigmentation Activity In Vitro
check_url/57760?article_type=v

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
check_url/57760?article_type=v

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
check_url/57760?article_type=v

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Read Article