In JoVE (1)
Other Publications (1)
Articles by Sang A Park in JoVE
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver Sang A Park*1,2, Young Ho Choe*1,2, Sung Hwan Lee3, Young-Min Hyun1,2 1Department of Anatomy, Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 3Department of Surgery, Yonsei University College of Medicine We established a novel surgical protocol for two-photon imaging of live mice liver with minimal invasion. With this technique, we identified the detailed structure of the liver during lipopolysaccharide-induced endotoxemia. We anticipate that this method may be utilized to determine the effectiveness of various reagents treatment to hepatic leukocyte migration.
Other articles by Sang A Park on PubMed
Neutrophil Extravasation Cascade: What Can We Learn from Two-photon Intravital Imaging? Immune Network. Dec, 2016 | Pubmed ID: 28035206 Immune cells (leukocytes or white blood cells) move actively and sensitively based on body conditions. Despite their important role as protectors inside the body, it is difficult to directly observe the spatiotemporal momentum of leukocytes. With advances in imaging technology, the introduction of two-photon microscopy has enabled researchers to look deeper inside tissues in a three-dimensional manner. In observations of immune cell movement along the blood vessel, vascular permeability and innate immune cell movements remain unclear. Here, we describe the neutrophil extravasation cascade, which were observed using a two-photon intravital imaging technique. We also provide evidence for novel mechanisms such as neutrophil body extension and microparticle formation as well as their biological roles during migration.