Articles by Timea Keller in JoVE
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells Meltem Avci-Adali1, Andreas Behring1, Heidrun Steinle1, Timea Keller1, Stefanie Krajeweski1, Christian Schlensak1, Hans P. Wendel1 1Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells.
Other articles by Timea Keller on PubMed
Neuropeptides Regulate Swimming Depth of Platynereis Larvae Proceedings of the National Academy of Sciences of the United States of America. Nov, 2011 | Pubmed ID: 22006315 Cilia-based locomotion is the major form of locomotion for microscopic planktonic organisms in the ocean. Given their negative buoyancy, these organisms must control ciliary activity to maintain an appropriate depth. The neuronal bases of depth regulation in ciliary swimmers are unknown. To gain insights into depth regulation we studied ciliary locomotor control in the planktonic larva of the marine annelid, Platynereis. We found several neuropeptides expressed in distinct sensory neurons that innervate locomotor cilia. Neuropeptides altered ciliary beat frequency and the rate of calcium-evoked ciliary arrests. These changes influenced larval orientation, vertical swimming, and sinking, resulting in upward or downward shifts in the steady-state vertical distribution of larvae. Our findings indicate that Platynereis larvae have depth-regulating peptidergic neurons that directly translate sensory inputs into locomotor output on effector cilia. We propose that the simple circuitry found in these ciliated larvae represents an ancestral state in nervous system evolution.
Optimized Conditions for Successful Transfection of Human Endothelial Cells with in Vitro Synthesized and Modified MRNA for Induction of Protein Expression Journal of Biological Engineering. 2014 | Pubmed ID: 24581116 The induction of protein synthesis by exogenous delivery of coding synthetic mRNA in desired cells is an auspicious strategy in the fields of basic cell biology, regenerative medicine, treatment of diseases, and reprogramming of cells. Here, we produced modified messenger RNA (mRNA) with reduced immune activation potential and increased stability and performed transfection experiments with different cells, HEK293 cells, BJ fibroblasts, and endothelial cells (ECs).