Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

June 2nd, 2023

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment (Video) | JoVE

01:25 min

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Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE

01:18 min

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Image Acquisition of Lipid Droplets and Analysis (Video) | JoVE

04:50 min

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Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra, Coimbra Health School

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Her demonstrerer vi en optimalisert BODIPY 493/503 fluorescensbasert protokoll for lipiddråpekarakterisering i levervev. Gjennom bruk av ortogonale projeksjoner og 3D-rekonstruksjoner muliggjør fluoroforen vellykket diskriminering mellom mikrovesikulær og makrovesikulær steatose og kan representere en komplementær tilnærming til de klassiske histologiske protokollene for vurdering av leversteatose.

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Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

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