Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

October 27th, 2023

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

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En protokol til evaluering af kvantitativ tumorcelledrab af Jurkat-celler, der udtrykker kimær antigenreceptor (CAR) rettet mod enkelt tumorantigen. Denne protokol kan bruges som screeningsplatform til hurtig optimering af CAR-hængselkonstruktioner inden bekræftelse i perifere blodafledte T-celler.

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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