Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

October 27th, 2023

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

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Ein Protokoll zur Bewertung der quantitativen Abtötung von Tumorzellen durch Jurkat-Zellen, die chimären Antigenrezeptor (CAR) exprimieren, der auf ein einzelnes Tumorantigen abzielt. Dieses Protokoll kann als Screening-Plattform für die schnelle Optimierung von CAR-Scharnierkonstrukten vor der Bestätigung in aus dem peripheren Blut gewonnenen T-Zellen verwendet werden.

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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