Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

October 27th, 2023

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04:00 min

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

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بروتوكول لتقييم القتل الكمي للخلايا السرطانية بواسطة خلايا جوركات التي تعبر عن مستقبلات المستضد الخيمري (CAR) التي تستهدف مستضد الورم المفرد. يمكن استخدام هذا البروتوكول كمنصة فحص للتحسين السريع لتركيبات مفصلات CAR قبل التأكيد في الخلايا التائية المشتقة من الدم المحيطي.

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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