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Immunology and Infection

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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Article DOI: 10.3791/50730-v • 08:31 min • October 15th, 2013
October 15th, 2013 •
1, 1, 1, 1
1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children, University of British Columbia

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Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.

Tags

Total Protein Extraction 2-D Gel Electrophoresis Burkholderia Species Protein Levels Pathogenic Mechanisms Mechanical Lysis Glass Beads Ethylenediamine Tetraacetic Acid Phenylmethylsulfonyl Fluoride Phosphate Buffered Saline Proteomic Studies Two-dimensional Gel Electrophoresis Isoelectric Focusing Acrylamide Gel Electrophoresis Silver Staining
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