Identification of Kinase-substrate Pairs Using High Throughput Screening

Courtney Reeks; Robert A. Screaton

doi:10.3791/53152

Journal

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生物学

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激酶底物对鉴定使用高通量筛选

Identification of Kinase-substrate Pairs Using High Throughput Screening

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生物学

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JoVE 杂志生物学

Identification of Kinase-substrate Pairs Using High Throughput Screening

激酶底物对鉴定使用高通量筛选

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8,088 Views

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11:13 min

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August 29, 2015

DOI:

10.3791/53152-v

DOI:

10.3791/53152-v

•

11:13 min

August 29, 2015

•

7 Views

Courtney Reeks¹, Robert A. Screaton^2,3

¹Children’s Hospital of Eastern Ontario Research Institute, ²Sunnybrook Research Institute,University of Toronto, ³Department of Biochemistry,University of Toronto

Summary

Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.