Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / A Method for Lineage Tracing of Corneal Cells…
JoVE Journal
Developmental Biology

这篇 内容 是开放权限的.

JoVE Journal Developmental Biology
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Journal (Developmental Biology)
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
Journal (Developmental Biology)
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
 

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Article DOI: 10.3791/53370-v • 07:48 min • December 18th, 2015
December 18th, 2015 •
*1, *2, 1, 2, 1, 3, 2, 1
1Department of Genetics and Developmental Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, 2Department of Ophthalmology, Hillel Yaffe Medical Center, 3Bioimging Center, Biomedical Core Facility, The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology
* These authors contributed equally

章节

总结概括
Automatic Translation

In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.

Tags

Lineage Tracing Corneal Cells Multi-color Fluorescent Reporter Mice Experimental Intervention Stem Cell Populations Mouse Genetics Fluorescent Microscopy Lineage Tracing Systems Ocular Surface R26R-Confetti Mouse Genetic System Adaptable Protocol Corneal Epithelium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter