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人类血小板的LC-MS分析作为研究线粒体代谢的平台

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

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环境科学

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JoVE 杂志环境科学

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

人类血小板的LC-MS分析作为研究线粒体代谢的平台

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11,191 Views

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06:04 min

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April 04, 2016

DOI:

10.3791/53941-v

DOI:

10.3791/53941-v

•

06:04 min

April 04, 2016

•

11170 Views

Andrew J. Worth*^1,2, Dylan M. Marchione*^2,3, Robert C. Parry^1,2, Qingqing Wang^2,3, Kevin P. Gillespie^2,3, Noelle N. Saillant⁴, Carrie Sims⁴, Clementina Mesaros^1,2, Nathaniel W. Snyder⁵, Ian A. Blair^1,2

¹Center for Cancer Pharmacology,University of Pennsylvania ²Center for Excellence in Environmental Toxicology,University of Pennsylvania ³Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania ⁴Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania ⁵A.J. Drexel Autism Institute,Drexel University

Summary

这里，我们表明分离的人血小板可以用作一个可访问的体外模型来研究的代谢适应响应于I抑制剂鱼藤酮的复合物。这种方法通过液相色谱 - 质谱法使用同位素示踪和相对定量，并且可以应用于各种研究设计的。

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