Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Haseong Kim; Kil Koang Kwon; Wonjae Seong; Seung-Goo Lee

doi:10.3791/54059

Journal

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生物学

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多酶筛选使用高通量基因酶的筛选系统

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生物学

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JoVE 杂志生物学

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

多酶筛选使用高通量基因酶的筛选系统

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8,644 Views

•

08:10 min

•

August 08, 2016

DOI:

10.3791/54059-v

DOI:

10.3791/54059-v

•

08:10 min

August 08, 2016

•

5 Views

Haseong Kim¹, Kil Koang Kwon¹, Wonjae Seong¹, Seung-Goo Lee^1,2

¹Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, ²Biosystems and Bioengineering Program,University of Science and Technology, Daejeon, South Korea

成績單

Summary

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).

多酶筛选使用高通量基因酶的筛选系统

成績單

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Summary

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