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Biochemistry

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JoVE Journal Biochemistry
En Protein Forberedelse Metode til High-throughput Identifikation af proteiner Samspil med en Nuclear Cofactor Brug LC-MS / MS-analyse
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En Protein Forberedelse Metode til High-throughput Identifikation af proteiner Samspil med en Nuclear Cofactor Brug LC-MS / MS-analyse

Article DOI: 10.3791/55077-v • 05:43 min • January 24th, 2017
January 24th, 2017 •
*1, *2, 3, 1, 3,4,5
1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences, Doshisha University
* These authors contributed equally

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Vi har etableret en fremgangsmåde til oprensning af coregulatoriske interaktion proteiner ved anvendelse af LC-MS / MS-system.

Tags

Biochemistry Protein interaktion LC-MS / MS proteinoprensning immunfældning nuklear receptor cofaktor transskription chromatin remodeling faktor proteinkompleks
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