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Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Article DOI: 10.3791/55689-v • 09:40 min • June 27th, 2017
June 27th, 2017 •
*1, *1, 1
1Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center
* These authors contributed equally

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DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.

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C-KIT Ligand Promoter Chromatin Immunoprecipitation TGF Beta 1 SMAD2 SMAD Binding Elements Cellular Biology Cytokine Induced Gene Transcription Transcription Factors Transcription Regulatory Proteins DNA Binding Sites Cell Culture Plates Fixation Solution Tissue Culture Medium Glycine Stop Fix Solution Cell Scraping Solution Centrifuge Samples
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