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JoVE Journal Neuroscience
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
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A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

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总结概括
Automatic Translation

We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.

Tags

Simple Cell-based Immunofluorescence Assay Autoantibody N-Methyl-D-Aspartate Receptor (NMDA Receptor) Blood Autoimmune Encephalitis Differential Diagnosis Neuro-psychiatric Symptoms Gelatin-coated Culture Plates HEK293 Cells Cell Culture Medium FBS (fetal Bovine Serum) NR1-GFP Plasmid Transfection Reagent
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