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En kvantitativ Dot Blot analysen for AAV titrering og bruk for funksjonell vurdering av Adeno-assosiert Virus montering-aktivere proteiner

Article DOI: 10.3791/56766-v • 14:49 min • June 12th, 2018
June 12th, 2018 •
1, 1, 1, 1,2
1Department of Molecular and Medical Genetics, Oregon Health & Science University, 2Department of Molecular Microbiology and Immunology, Oregon Health & Science University

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Dette manuskriptet detaljer en enkel prikk blot analysen for kvantifisering av adeno-assosiert virus (AAV) titers og dens anvendelse å studere rollen til montering-aktivere proteiner (AAPs), en roman klasse av ikke-strukturell virale proteiner i alle AAV serotyper, fremme montering av capsids fra beslektet og heterologous AAV serotyper.

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Biologi problemet 136 Adeno-assosiert virus (AAV) aktivering av montering protein (AAP) cross-complementation kvantitativ dot blot analysen viral titer genterapi
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