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Developmental Biology

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JoVE Journal Developmental Biology
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

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Single-cell Transcriptomic Analyses Mouse Pancreatic Endocrine Cells Pancreatic Islet Lineage Differentiation Maturation Regeneration High Quality Single Cell RNA Sequencing Data Therapy Of Diabetes Pancreatic Endocrine Related Diseases Transcriptomic Analysis Pathological Conditions Visual Demonstration Narrow Bile Duct Perfusion Embryonic Day 17.5 Embryos Abdominal Cavity Elbow Tweezers Visceral Tissue Six Centimeter Black Bottom Dish Cold PBS Forceps Pancreas Detachment 20 Milliliter Vial Five Milliliters Of Cold Collagenase Solution Post Natal Day Zero To 15 Mice Post Natal Day 18 To 60 Mice Xiphoid Bowel Extraction
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