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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Article DOI: 10.3791/63923-v • 08:55 min • July 12th, 2022
July 12th, 2022 •
1, 1, *1, *1,2,3
1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
* These authors contributed equally

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The present protocol describes high-resolution cryo-electron tomography remote data acquisition using Tomo5 and subsequent data processing and subtomogram averaging using emClarity. Apoferritin is used as an example to illustrate detailed step-by-step processes to achieve a cryo-ET structure at 2.86 Å resolution.

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Cryo-electron Tomography Remote Data Collection Subtomogram Averaging Cellular Specimens Native State High Resolution CROW ET Sub Atom Averaging Tomo Five Software TEM Server Pc Image Acquisition Parameters Presets Exposure Settings Atlas Session Preferences Storage Path Output Format Screening Close Call Valves Start Button Targets Grid Selection Sample Loading Image Shift Calibration
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