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Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Article DOI: 10.3791/3599-v • 12:19 min • May 27th, 2012
May 27th, 2012 •
1,2,3, 4,5,6, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, 3Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, 4École Centrale Marseille, Technopôle de Château-Gombert, 5Institut Fresnel, Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University

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Summary
Automatic Translation

Multiple-Target Tracing is a homemade algorithm developed for tracking individually labeled molecules within the plasma membrane of living cells. Efficiently detecting, estimating and tracing molecules over time at high-density provide a user-friendly, comprehensive tool to investigate nanoscale membrane dynamics.

Tags

Molecular Diffusion Plasma Membrane Multiple-target Tracing (MTT) Cellular Membranes Single-molecule Level Single-particle Tracking (SPT) Videomicroscopy Millisecond Resolution Nanometric Resolution Membrane Organization Cell Receptors Localization Mobility Confinement Interactions Experimental Optimization Cell Labeling Labeling Density Molecular Dynamics Algorithmic Issues Peaks Detection Estimation And Reconnection Image Analysis Deflation After Detection Monte-Carlo Simulations Labeling Density Noise Value
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