JoVE Journal
Immunology and Infection
Immunology and Infection
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Summary
Please note that all translations are automatically generated.
This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.