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Journal / Bioengineering / Vurdere kollagen og Elastin press-avhengige…
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Bioengineering

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JoVE Journal Bioengineering
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Vurdere kollagen og Elastin press-avhengige Microarchitectures i Live, menneskelig motstand arterier av etiketten-fri fluorescens mikroskopi

Article DOI: 10.3791/57451-v • 09:58 min • April 9th, 2018
April 9th, 2018 •
1, 2, 2, 1,3
1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital

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Vi beskriver samtidig mekanisk testing og 3D-bildebehandling av arterieveggen av isolert, live menneskelig motstand arterier og Fiji og Ilastik bilde analyser for kvantifisering av elastin og kollagen romlig organisering og volum tettheter. Vi diskuterer bruken av disse dataene i matematiske modeller av arterieveggen mekanikk.

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Bioteknologi problemet 134 to-fotonet eksitasjon fluorescens mikroskopi ekstracellulær matrix bilde analyser motstand arteriene Ilastik Fiji mikrostruktur mekanikk matematisk modellering
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