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Identifiering av posttranslationella modifieringar av växtproteinkomplex
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Identifiering av posttranslationella modifieringar av växtproteinkomplex

Article DOI: 10.3791/51095-v • 10:07 min • February 22nd, 2014
February 22nd, 2014 •
1, 2, 2, 1,2, 3, 1
1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University

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Vi beskriver här ett protokoll för rening och karakterisering av växtproteinkomplex. Vi visar att genom immunoprecipitating ett enda protein i en komplex, så att vi kan identifiera dess post-translationella modifieringar och dess interagerande partners.

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Växtbiologi växt-mikrob interaktioner proteinkomplex rening masspektrometri proteinfosforylering Prf kraftuttag AvrPto AvrPtoB
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