Tissue Triage and Freezing for Models of Skeletal Muscle Disease

Hui Meng; Paul M.L. Janssen; Robert W. Grange; Lin Yang; Alan H. Beggs; Lindsay C. Swanson; Stacy A. Cossette; Alison Frase; Martin K. Childers; Henk Granzier; Emanuela Gussoni; Michael W. Lawlor

doi:10.3791/51586

Journal

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Biología

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Triage de tejidos y de congelación para los modelos de la enfermedad del músculo esquelético

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

JoVE Revista
Biología

This content is Free Access.

JoVE Revista Biología

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

Triage de tejidos y de congelación para los modelos de la enfermedad del músculo esquelético

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39,879 Views

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05:58 min

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July 15, 2014

DOI:

10.3791/51586-v

DOI:

10.3791/51586-v

•

05:58 min

July 15, 2014

•

43 Views

Hui Meng¹, Paul M.L. Janssen², Robert W. Grange³, Lin Yang⁴, Alan H. Beggs⁵, Lindsay C. Swanson⁵, Stacy A. Cossette^1,6, Alison Frase⁷, Martin K. Childers⁸, Henk Granzier⁹, Emanuela Gussoni⁵, Michael W. Lawlor¹

¹Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine,Medical College of Wisconsin, ²Department of Physiology and Cell Biology,The Ohio State University, ³Department of Human Nutrition, Foods and Exercise,Virginia Tech, ⁴Division of Biomedical Informatics, Department of Biostatistics, Department of Computer Science,University of Kentucky, ⁵Division of Genetics and Genomics, The Manton Center for Orphan Disease Research,Boston Children’s Hospital, Harvard Medical School, ⁶Cure Congenital Muscular Dystrophy, ⁷Joshua Frase Foundation, ⁸Department of Rehabilitation Medicine,University of Washington, ⁹Department of Physiology,University of Arizona

Summary

The analysis of skeletal muscle tissues to determine structural, functional, and biochemical properties is greatly facilitated by appropriate preparation. This protocol describes appropriate methods to prepare skeletal muscle tissue for a broad range of phenotyping studies.