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Journal / Genetics / 使用染色质免疫沉淀和高通量测序（ChIP-seq）发现沙门氏菌生物膜中的CsgD监管目标…
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Genetics
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JoVE Journal Genetics
使用染色质免疫沉淀和高通量测序（ChIP-seq）发现沙门氏菌生物膜中的CsgD监管目标
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使用染色质免疫沉淀和高通量测序（ChIP-seq）发现沙门氏菌生物膜中的CsgD监管目标

Article DOI: 10.3791/60736-v • 13:48 min • January 18th, 2020
January 18th, 2020 •
1,2, 3, 1,2
1Vaccine and Infectious Disease Organization-International Vaccine Centre, 2Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, 3Department of Cell Biology and Genetics, Shenzhen University Health Science Center

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染色质免疫沉淀与下一代测序（ChIP-seq）相结合，是一种用于建立转录因子与其控制的基因组序列之间的相互作用的方法。该协议概述了使用细菌生物膜执行ChIP-seq的技术，以沙门氏菌肠杆菌菌膜为例。

Tags

遗传学， 问题 155， 染色质免疫沉淀， ChIP-seq， 下一代测序， 生物膜， 细菌， 沙门氏菌
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