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Ingegneria genomica delle cellule B umane primarie utilizzando CRISPR/Cas9
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Ingegneria genomica delle cellule B umane primarie utilizzando CRISPR/Cas9

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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Qui forniamo un protocollo dettagliato passo-passo per l'ingegneria del genoma basata su CRISPR / Cas9 delle cellule B umane primarie per knockout genico (KO) e knock-in (KI) per studiare le funzioni biologiche dei geni nelle cellule B e lo sviluppo di terapie a cellule B.

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Biologia Numero 165 CRISPR/Cas9 ingegneria del genoma AAV ricombinante editing genico cellule B umane primarie
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