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JoVE Journal Neuroscience
Påvisning af unormalt prionprotein ved immunhistokemi
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Påvisning af unormalt prionprotein ved immunhistokemi

Article DOI: 10.3791/64560-v • 06:38 min • May 5th, 2023
May 5th, 2023 •
1,2, 1, 2, 2, 2, 3, 2, 1, 1, 1, 1, 1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

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Immunmærkning af unormalt prionprotein ved hjælp af immunhistokemiske protokoller kræver specifikke prøve- og anti-PrP-antistofpræparationsmetoder. Denne protokol beskriver de vigtigste trin i epitopmaskering for at sikre korrekt PrP-immunmærkning og for at minimere ikke-specifik baggrundsfarvning. Denne tilgang overvejer også biosikkerhedsforanstaltninger ved udførelse af immunhistokemiske undersøgelser med de prioninficerede væv.

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Neurovidenskab nummer 195
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