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JoVE Journal Neuroscience
Isolering og dyrkning af vestibulær og spiralganglionsomata fra neonatale gnavere til patch-clamp-optagelser
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Isolering og dyrkning af vestibulær og spiralganglionsomata fra neonatale gnavere til patch-clamp-optagelser

Article DOI: 10.3791/64908-v • 11:05 min • April 21st, 2023
April 21st, 2023 •
1, 1, 1, 1, 1, 1
1Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program, University of Southern California

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Her præsenteres metoder, der giver detaljerede instruktioner til dissekering, dissociering, dyrkning og patch-clamp-optagelse fra vestibulær ganglion og spiralganglionneuroner i det indre øre.

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Neurovidenskab udgave 194 somata neonatale gnavere patch-clamp optagelser indre øreganglion neuroner ionkanaler neurotransmitterreceptorer cellediversitet dissekere dissociere dyrkning bipolære neuroner kortvarig dyrkning indre øre somata plating spiral ganglion neuroner helcelle patch-clamp optagelser perforeret-patch konfiguration spænding-clamp optagelser hyperpolarisering-aktiveret cyklisk nukleotid-gated (HCN)-medieret strømme rupt-patch konfiguration rupt-patch konfiguration cellulære neuroner ruptured-patch konfiguration cellulære neuroner helcelle patch-optagelser Processer G-protein koblede receptorer
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