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Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

Article DOI: 10.3791/789-v • 10:39 min • June 27th, 2008
June 27th, 2008 •
1, 1, 2, 1,3
1Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School, 2Department of Cell Biology and Genetics, Erasmus University Medical Center, 3Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School

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This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.

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Isolation Early Hematopoietic Stem Cells Murine Yolk Sac AGM Mouse Embryo Blood System Fetal Liver Bone Marrow Microdissection Flow Cytometric Sorting Pure Population Stem Cell Pool Functional Differences Genetic Models Video Demonstration FACS Plot Functional Assays Colony Assays Bone Marrow Transplants
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