Materials
Name | Company | Catalog Number | Comments |
Ethanol | Fisher | 32221 | |
Anhydrous dimethylformamide (DMF) | Sigma | 270547 | |
Ammonium hydroxide 50% (v/v) aqueous solution | Alfa Aesar | 35574 | diluted to 30% (v/v) with pure water |
TEOS | Sigma | 13190-3 | |
3-Aminopropyltriethoxysilane (APTES) | Sigma | A3648 | |
5-(and-6)-carboxyfluorescein, succinimidyl ester (FAM-SE) | Invitrogen | C1311 | |
6-carboxytetramethylrhodamine, succinimidyl ester (TAMRA-SE) | Invitrogen | C1171 | |
Sodium phosphate monobasic (0.2 M) | Sigma Aldrich | S-9638 | |
Sodium phosphate dibasic (0.2 M) | Sigma Aldrich | S-0876 | |
NaOH | Sigma Aldrich | S8045 | |
Trypsin/EDTA | Sigma Aldrich | T4174 | |
Penicillin/Streptomycin | Sigma Aldrich | P0781 | |
PBS | Sigma Aldrich | D8537 | |
DMEM | Sigma Aldrich | D6046 | |
FBS | Source Bioscience | Batch-213-101992 | |
L-Glutamine | Sigma Aldrich | G7513 | |
Lipofectamine 2000 | Invitrogen | 11668-019 | |
Optimem | Invitrogen | 11058-021 | |
LysoTracker Red | Invitrogen | L-7528 | |
Draq5 | Biostatus Ltd | DR50050 | |
Nitrogen gas | BOC | ||
DCM | Sigma Aldrich | 320269 | |
TFA | Sigma Aldrich | T6508 | |
Confocal microscope | Leica TCS-SP | equipped with argon and krypton lasers and a 63X 0.9NA water immersion lens | |
UV light | UVLS28 UVP, USA | ||
Stirrer plate | SB161-3 Jencons-PLS | ||
pH meter | Jenway model 3510 | ||
Rotary Evaporator | Buchi Rotary Evaporator R200 | ||
Centrifuge (nanosensors) | Hermle Z300 | ||
Centrifuge (cell culture) | Thermo Scientific Heraeus Biofuge Primo | ||
Vortex | Whirlimixer Fisherbrand | ||
Ultrasonicator | FB11021 Fisherbrand | ||
Aluminum sheet | Nottingham University | ||
35mm cell culture plate | Iwaki | 3000035 | |
10 ml syringe | Becton Dickenson | ||
3T3 Fibroblast cells | European Collection of Cell Cultures | ||
PLGA | Lakeshore Biomaterials | 7525 DLG 7E | |
Pyridinium formate | Sigma Aldrich | P8535 | |
Trypan blue | Sigma Aldrich | T8154 | |
Sodium phosphate monobasic | Sigma Aldrich | S9638 | |
Sodium phosphate dibasic | Sigma Aldrich | S5136 | |
HCl | Sigma Aldrich | 320331 |
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