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Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
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Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR

Article DOI: 10.3791/1974-v • 11:22 min • May 4th, 2010
May 4th, 2010 •
1, 1, 1, 1
1Department of Entomology, The Ohio State University

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Quantitative real-time PCR (qRT-PCR) is an effective tool to diagnose mRNA levels in different insect tissues and developmental stages. In this report we show the use of qRT-PCR to ascertain mRNA levels in different larval tissues and developmental stages of the invasive insect species, emerald ash borer.

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Gene Expression Emerald Ash Borer Agrilus Planipennis Quantitative Real-time PCR EAB Invasive Pest Ash Trees Molecular Knowledge Physiological Processes Tissue Level MRNA Levels Larval Tissues Developmental Stages Peritrophin Gene AP-PERI1 Ribosomal Protein AP-RP1 Peritrophic Membrane/matrix Insect Gut
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