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Immunology and Infection

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Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Article DOI: 10.3791/50639-v • 10:18 min • December 10th, 2013
December 10th, 2013 •
1, 1, 1,3, 2
1Department of Dental Materials, College of Dentistry, University of Oklahoma Health Sciences Center, 2Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, 3Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University

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A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.

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Biofilms Concurrent Quantification Cellular Components Extracellular Components Biofilm Investigation Confocal Laser Scanning Microscopy CLSM Fluorescent Dyes Quantification Multiple Fluorochromes Methodology 3-dimensional Distribution Biofilm Growth Staining Imaging Structural Analysis Statistical Analysis Streptococcus Mutans Resin Composites Mouthwashes Water Control Group Fluorochromes Staining Extracellular Polymeric Substances Proteins Nucleic Acids
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