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Bioengineering

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JoVE Journal Bioengineering
Samtidig kvantificering af udvalgte kynureniner analyseret af flydende kromatografi-massespektrometri i medium indsamlet fra kræftcellekulturer
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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Journal (Bioengineering)
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
 
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Samtidig kvantificering af udvalgte kynureniner analyseret af flydende kromatografi-massespektrometri i medium indsamlet fra kræftcellekulturer

Article DOI: 10.3791/61031-v • 10:38 min • May 9th, 2020
May 9th, 2020 •
1, 1, 1
1Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin

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Beskrevet her er en protokol til bestemmelse af fire forskellige tryptofan metabolitter genereret i kynureninvejen (kynurenin, 3-hydroxykynurenin, xanthurensyre, 3-hydroxyanthranilic syre) i mediet indsamlet fra kræftcellekulturer analyseret af flydende kromatografi kombineret med en enkelt firdoblet massespektrometri.

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Bioengineering Problem 159 væskekromatografi massespektrometri tryptofanmetabolitter kynurenin 3-hydroxykynurenin xanthurensyre 3-hydroxyanthranilic syre kræftceller prøveforberedelse
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