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Genetics

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JoVE Journal Genetics
CRISPR-Cas9-Medierad genomredigering i den fintrådiga Ascomycete Huntiella omanensis
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CRISPR-Cas9-Medierad genomredigering i den fintrådiga Ascomycete Huntiella omanensis

Article DOI: 10.3791/61367-v • 07:25 min • June 9th, 2020
June 9th, 2020 •
1,2, 1,2
1Department of Biochemistry, Genetics & Microbiology, University of Pretoria, 2Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

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CRISPR-Cas9-genomredigeringssystemet är en lättanvänd genomredigerare som har använts i modell- och icke-modellarter. Här presenterar vi en proteinbaserad version av detta system som användes för att införa en tidig stopp kodon i en parning gen av en icke-modell fintrådiga ascomycete svamp.

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Genetik Genomredigering CRISPR-Cas9 RNP Transformationer Sexuell reproduktion Svampar Huntiella omanensis
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