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Visualisering af DNA Reparation Proteiner Interaktion ved Immunofluorescence
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Journal (Biology)
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
Journal (Biology)
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
 
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Visualisering af DNA Reparation Proteiner Interaktion ved Immunofluorescence

Article DOI: 10.3791/61447-v • 07:55 min • June 26th, 2020
June 26th, 2020 •
1,2, 1,2
1Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, 2Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

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Efter DNA-skader aktiverer humane celler vigtige reparationsveje for at genoprette integriteten af deres genom. Her beskriver vi metoden til indirekte immunfluorescens som et middel til at detektere DNA-reparationsproteiner, analysere deres rumlige og tidsmæssige rekruttering og hjælpe med at afhøre proteinproteininteraktion på steder med DNA-skader.

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Biologi Immunofluorescence co-lokalisering nuklear foci bestråling-induceret foci DNA-skader reparation
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