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Journal / Neuroscience / Afledning, ekspansion, kryopræservering og…
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Neuroscience

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JoVE Journal Neuroscience
Afledning, ekspansion, kryopræservering og karakterisering af hjernemikrobielle endotelceller fra menneskeskabte pluripotente stamceller
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Afledning, ekspansion, kryopræservering og karakterisering af hjernemikrobielle endotelceller fra menneskeskabte pluripotente stamceller

Article DOI: 10.3791/61629-v • 08:04 min • November 19th, 2020
November 19th, 2020 •
1,2,3, 1,2,3,4, 1,3,4,5
1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital

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Denne protokol beskriver en tilpasset metode til at udlede, udvide og kryopreserve hjernens mikrovaskulære endotelceller opnået ved at differentiere humane inducerede pluripotente stamceller og til at studere blodhjernebarriereegenskaber i en ex vivo-model.

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Neurovidenskab Problem 165 Human induceret pluripotente stamceller hjernens mikrovaskulære endotelceller blodhjernebarriere trans-endotel elektrisk modstand transportaktivitet mikrovaskulær skizofreni bipolar lidelse passaging ekspansion kryopræservering
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