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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analyse til undersøgelse af Host-Patogen Interaktioner
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Journal (Immunology and Infection)
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Journal (Immunology and Infection)
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
 
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High-Throughput Transcriptome Analyse til undersøgelse af Host-Patogen Interaktioner

Article DOI: 10.3791/62324-v • 14:58 min • March 5th, 2022
March 5th, 2022 •
1,2, 3, 2,3, 3, 2,3, 2,3,4
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School, University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, 4Hospital Israelita Albert Einstein

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Den protokol, der præsenteres her, beskriver en komplet pipeline til at analysere RNA-sekventering af transskriptionomdata fra rå læser til funktionel analyse, herunder kvalitetskontrol og forbehandlingstrin til avancerede statistiske analytiske tilgange.

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Immunologi og infektion Udgave 181 sekventering med høj gennemløb RNA-seq værtspatogeninteraktion differentierede gener med udtrykte gener funktionel analyse molekylær grad af forstyrrelse af prøver
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