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Journal / Bioengineering / A Method for Ovarian Follicle Encapsulation and…
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Bioengineering

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A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

Article DOI: 10.3791/2695-v • 12:37 min • March 15th, 2011
March 15th, 2011 •
1, 2, 2,3,4, 1,4,5
1Institute for BioNanotechnology in Advanced Medicine, Northwestern University, 2Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research, Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 5Department of Chemical and Biological Engineering, Northwestern University

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A new method for ovarian follicle encapsulation in a 3D fibrin-alginate interpenetrating network is described. This system combines structural support with proteolytic degradation to support the development of immature follicles to produce mature oocytes. This method may be applied to culture cell aggregates to maintain cell-cell contacts without limiting expansion.

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Ovarian Follicle Encapsulation Culture Proteolytically Degradable 3 Dimensional System In Vitro Follicle Techniques Hydrogels Native Ovarian Environment 3D Follicular Architecture Cell-cell Interactions Paracrine Signaling Alginate Inert Algae-derived Polysaccharide Gelation Calcium Ions Developmental Competence Degradable Fibrin-alginate Interpenetrating Network (FA-IPN) Dynamic Mechanical Environment
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