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Journal / Biology / Hsp104의 정화, 단백질 Disaggregase
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Hsp104의 정화, 단백질 Disaggregase
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Hsp104의 정화, 단백질 Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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여기서 우리는 매우 적극적인 Hsp104, hexameric AAA + 단백질 효모, 단백질 disaggregation에 커플 ATP의 가수 분해로부터의 정화를 위해 프로토콜을 설명합니다. 이 제도는의 친화력 정화를위한 His6 - 태그 구축을 악용

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분자 생물학 제 55 신경 과학 Hsp104 AAA + disaggregase 열 충격 아밀로이드 prion
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