Production of Double-stranded DNA Ministrings

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Production of Double-stranded DNA Ministrings

DOI:

10.3791/53177-v

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06:12 min

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February 29, 2016

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Shirley Wong, Peggy Lam, Nafiseh Nafissi, Steven Denniss, Roderick Slavcev

¹School of Pharmacy,University of Waterloo, ²Sgd Health Innovation

Chapters

00:05Title
00:39Preparation of Media and Cultures
01:58Growth Phase
03:19Ministring Production and Harvesting
04:31Results: Double-stranded Ministring Production
05:13Conclusion

Summary

Automatic Translation

The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.

Production of Double-stranded DNA Ministrings

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Chapters

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