Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

This content is Open Access.

Production of Double-stranded DNA Ministrings

Production of Double-stranded DNA Ministrings

Article DOI: 10.3791/53177-v 06:12 min February 29th, 2016
February 29th, 2016



The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.


DNA Ministrings Gene Delivery DNA Vector In Vivo Approach LB Agar Ampicillin Media Preparation Overnight Culture W3NN PDMS Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter