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JoVE Journal
Biology

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Production of Double-stranded DNA Ministrings
 

Production of Double-stranded DNA Ministrings

Article DOI: 10.3791/53177
February 29th, 2016

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Summary February 29th, 2016

The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.

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