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JoVE Journal
Biology

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Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
 

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Article doi: 10.3791/3340
October 27th, 2011

Summary October 27th, 2011

Here we describe a method for preparation of both single read and paired end Illumina mRNA-Seq sequencing libraries for gene expression analysis based on T7 linear RNA amplification. This protocol requires only 10 nanograms of starting total RNA and generates highly consistent libraries representing whole transcripts.

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