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JoVE Journal Biochemistry
Bewertung von Protein-Protein-Wechselwirkungen mit einer On-Membrane-Verdauungstechnik
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Bewertung von Protein-Protein-Wechselwirkungen mit einer On-Membrane-Verdauungstechnik

Article DOI: 10.3791/59733-v • 07:07 min • July 19th, 2019
July 19th, 2019 •
*1, *2, 1, 2, 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine
* These authors contributed equally

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Hier stellen wir ein Protokoll für eine On-Membran-Verdauungstechnik zur Vorbereitung von Proben für die Massenspektrometrie vor. Diese Technik erleichtert die bequeme Analyse von Protein-Protein-Wechselwirkungen.

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Biochemie Ausgabe 149 Immunpräzipitation LC/MS/MS Proteomik Proteininteraktion PVDF-Membran Screening
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